Journal of Biomedical and Engineering Research
ISSN: 3065-8780 | DOI: 10.64030/3065-8780
Blessing K Gana, Olufunmilayo H Akanya and Evans C Egwim
The aim of this study was to determine the amino acid composition of cassava seed protein concentrate. Preparation of the protein concentrate from defatted cassava seed flour was carried out by isoelectric precipitation extraction method. Amino acid analysis was carried out using automated amino acid analyzer. The amino acid score was calculated using WHO provisional scoring pattern for preschool children (1-2years). The obtained result showed that a total of 18 amino acids were identified in cassava seeds protein concentrate consisting of 9 essential amino acids and 9 non-essential amino acids. The total amino acid content was 79.88 ± 7.99 g/100g.Generally, glutamic acid (13.78 ± 2.15 g/100g) was the highest amino acid while tryptophan (0.83 ± 0.99 g/100g) was found to be the least amino acid. The concentration of the total non-essential amino acid was 48.75 ± 3.49 g/100g, for essential amino acids, the total essential amino acids was 29.76 ± 2.56 g/100g. The concentration of total sulphur-containing amino acid was 2.90 ± 0.85 g/100g while concentration of total aromatic amino acid was 7.22 ± 0.02 g/100g. Lysine was the limiting amino acid (amino acid score = 0.88). The findings suggest that cassava seed protein concentrate could be a rich source of amino acids for human and animal diet and could be incorporated into food therefore improving protein quality in diets and alleviating protein energy malnutrition.