CME Journal of Medical Microbiology
ISSN: 3070-3638 | DOI: 10.64030/3070-3638
Halah Dawood Salman
Background: Patients in post chemotherapy susceptible to have variable complications or infections, mucositis and oral ulcers one of these changes of oral flora as chemotherapy predispose for these infections. Objective: The current study aimed to investigate Escherchia coli isolates as a causative agent of oral ulcer infections in post chemotherapy patients, as well as to establish the existence and genotype of virulence genes (pap c, cnf1, fimH, blaOXA and hly beta ) in E. coli isolates, moreover to detect the relationship of these local isolates to internationally isolates through alignments of aimed genes sequenceing and phylogentic trees of these isolates.
Subjects and Methods: In this study, 150 clinical samples were collected from oral ulcer infections in post chemotherapy patients for isolation of E. coli that identified and diagnosed biochemically and molecularly using specific primers to detect virulence genes (pap c, cnf1, fimH, blaOXA and hly beta ) in E. coli isolates, the study also included 25 apparently healthy people as a (control group) for collecting oral swabs from oral ulcer infections of post chemotherapy patients. Construction of phylogenetic trees was included.
Results: The study found only 43 ( 28.6 %) of patients infected with E.coli while the rest 107 ( 71.3 %) of patients not infected with E.coli, only 10 (23.2 % ) of the tested isolates have pap c gene , 11 isolates (25.5% ) have cnf1 gene, 10 isolates ( 23.2 %) have fimH gene , 5 isolates (11.6% ) have blaOXA gene and 12 isolates ( 27.9 %) have hly beta gene, moreover, phylogenetic tree of locally gene sequences that required for E.coli isolates showed identical in high percentage comparison to different countries according to the sequence of nucleotide bases derived from GenBank database, control group revealed negative results for the presence of E.coli.
Conclusions: The study concluded that E.coli isolated from oral ulcer of patients in post chemotherapy have pap C, cnf1, fimH, blaOXA and hly beta genes which complicate health status of cancer patients through bacterial oral mucositis, moreover, phylogenetic analysis of these strains showed identical ratios according to the sequence of nucleotide bases of different countries derived from GenBank database at NCBI.